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Wham clusters all of the candidate breakpoints and rounds their positions to the nearest tenth base pair. The posture with the very best read through assist is described. When the soft-clipped consensus sequence is often aligned towards the putative breakpoint location using the Smith-Waterman algorithm, the breakpoint is even more refined to The s
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Wham is created for paired-conclude Illumina libraries with regular insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, break up read through mapping, comfortable-clipping, option alignment and consensus sequence primarily based proof to predict SV breakpoints with one-nucleotide precision. Wham generates a put together pileup (c